abstract：:Computer simulation methods are increasingly being used to study possible conformations and dynamics of structural motifs in RNA. Recent results of molecular dynamics simulations and continum solvent studies of RNA structures and RNA-ligand complexes show promising agreement with experimental data. Combined with the o...

journal_title：Current opinion in structural biology

authors： Zacharias M

更新日期：2000-06-01 00:00:00

abstract：:Self-compartmentalizing proteases, such as the proteasome and several prokaryotic energy-dependent proteases, are designed to act in the crowded environment of the cell. Proteins destined for degradation are recognized and unfolded by regulatory subcomplexes that invariably contain ATPase modules, before being translo...

journal_title：Current opinion in structural biology

authors： Zwickl P,Baumeister W,Steven A

更新日期：2000-04-01 00:00:00

abstract：:Newly solved chaperone structures include the thermosome, a group II chaperonin, and a small heat-shock protein. Novel ideas on chaperone mechanism are presented in the forced unfolding hypothesis of GroEL action. Structures of chaperone-pilin complexes reveal the mechanism of chaperone interaction in bacterial pilus ...

journal_title：Current opinion in structural biology

authors： Saibil H

更新日期：2000-04-01 00:00:00

abstract：:The past two years have seen the extension of our knowledge on the cellular prion protein structure with new NMR data on both the hamster and human proteins. In addition, the folding dynamics of two cellular prion proteins have been elucidated. There are now several examples of recombinant prion proteins that are able...

journal_title：Current opinion in structural biology

authors： Jackson GS,Clarke AR

更新日期：2000-02-01 00:00:00

abstract：:The winged helix proteins constitute a subfamily within the large ensemble of helix-turn-helix proteins. Since the discovery of the winged helix/fork head motif in 1993, a large number of topologically related proteins with diverse biological functions have been characterized by X-ray crystallography and solution NMR ...

journal_title：Current opinion in structural biology

authors： Gajiwala KS,Burley SK

更新日期：2000-02-01 00:00:00

abstract：:Recently solved structures and proposed models have helped to reveal the structural characteristics of the beta-propeller fold, as well as the features that contribute to its high rigidity and stability. Possible strategies for identifying beta-propeller proteins in newly characterised sequences are helping to overcom...

journal_title：Current opinion in structural biology

authors： Fülöp V,Jones DT

更新日期：1999-12-01 00:00:00

abstract：:Recent advances in ab initio direct methods have enabled the solution of crystal structures of small proteins from native X-ray data alone, that is, without the use of fragments of known structure or the need to prepare heavy-atom or selenomethionine derivatives, provided that the data are available to atomic resoluti...

journal_title：Current opinion in structural biology

authors： Usón I,Sheldrick GM

更新日期：1999-10-01 00:00:00

abstract：:Current knowledge about the determinants of beta-sheet formation has been notably improved by the structural and kinetic analysis of model peptides, by mutagenesis experiments in proteins and by the statistical analysis of the protein structure database (Protein Data Bank; PDB). In the past year, several peptides comp...

journal_title：Current opinion in structural biology

authors： Lacroix E,Kortemme T,Lopez de la Paz M,Serrano L

更新日期：1999-08-01 00:00:00

abstract：:Large ribozymes fold on a 'glacial' timescale compared to the folding of their protein counterparts. The sluggish folding exhibited by large RNAs results from the formation of kinetically trapped, misfolded intermediates, which are nonessential features of the folding mechanism. Newly developed mutant ribozymes that a...

journal_title：Current opinion in structural biology

authors： Treiber DK,Williamson JR

更新日期：1999-06-01 00:00:00

abstract：:Gamma-tubulin is as ubiquitous in eukaryotes as alpha- and beta-tubulin. Rather than forming part of the microtubule wall, however, gamma-tubulin is involved in microtubule nucleation. Although gamma-tubulin concentrates at microtubule-organizing centers, it also exists in a cytoplasmic complex whose size and complexi...

journal_title：Current opinion in structural biology

authors： Wiese C,Zheng Y

更新日期：1999-04-01 00:00:00

abstract：:Dynamin is an important component of membrane recycling at the plasma membrane and, potentially, within the cell. The role of dynamin in clathrin-mediated endocytosis has been based on numerous endocytosis assays, as well as on the discovery and gross characterization of the assembled spiral structure of dynamin. Rece...

journal_title：Current opinion in structural biology

authors： Hinshaw JE

更新日期：1999-04-01 00:00:00

abstract：:Investigating the in vitro refolding of proteins that naturally reside in biological membranes is a notoriously difficult task. Biophysical studies on model systems are beginning to provide a sound physical basis for membrane protein folding that should help to alleviate this problem. Highlights of these studies inclu...

journal_title：Current opinion in structural biology

authors： Booth PJ,Curran AR

更新日期：1999-02-01 00:00:00

abstract：:The structure of tubulin has recently been determined by electron crystallography, paving the way for a clearer understanding of the unique properties of tubulin that allow its varied functions within the cell. Some of the ongoing work on tubulin can be interpreted in terms of its structure, which can serve to guide f...

journal_title：Current opinion in structural biology

authors： Downing KH,Nogales E

更新日期：1998-12-01 00:00:00

abstract：:Terpenoid cyclases catalyze remarkably complex cyclization cascades that are initiated by the formation of a highly reactive carbocation in a polyisoprene substrate. Recent crystal structures of terpenoid cyclases show how these enzymes provide a template for binding and stabilizing the flexible substrate in the preci...

journal_title：Current opinion in structural biology

authors： Lesburg CA,Caruthers JM,Paschall CM,Christianson DW

更新日期：1998-12-01 00:00:00

abstract：:The three-dimensional structures of membrane proteins are essential for understanding their functions, interactions and architectures. Their requirement for lipids has hampered structure determination by conventional approaches. With optimized samples, it is possible to apply solution NMR methods to small membrane pro...

journal_title：Current opinion in structural biology

authors： Marassi FM,Opella SJ

更新日期：1998-10-01 00:00:00

abstract：:The surfaces of all microbes are 'sugar coated' with molecules such as lipopolysaccharides in Gram-negative bacteria, capsular polysaccharides in bacteria, lipoarabinomannans in mycobacteria and lipophosphoglycan in Leishmania. The basic structures of these glycoconjugates are known and, in the case of pathogens, they...

journal_title：Current opinion in structural biology

authors： Mengeling BJ,Turco SJ

更新日期：1998-10-01 00:00:00

abstract：:The exponential growth in the amount of genomic data published in recent years has led to increased efforts in analysing genomes for the presence of repeated sequences, which has in turn fostered the development of novel repeat recognition methods. This has resulted in a deepened understanding of the importance and ab...

journal_title：Current opinion in structural biology

authors： Heringa J

更新日期：1998-06-01 00:00:00

abstract：:Intermediate filaments are built from one to several members of a multigene family encoding fibrous proteins that share a highly conserved hierarchic assembly plan for the formation of multistranded filaments from distinctly structured extended coiled coils. Despite the rather low primary sequence identity, intermedia...

journal_title：Current opinion in structural biology

authors： Herrmann H,Aebi U

更新日期：1998-04-01 00:00:00

abstract：:Specific, noncovalent binding of biomolecules can only be understood by considering structural, thermodynamic, and kinetic issues. The theoretical foundations for such analyses have been clarified in the past year. Computational techniques for both particle-based and continuum models continue to improve and to yield u...

journal_title：Current opinion in structural biology

authors： McCammon JA

更新日期：1998-04-01 00:00:00

abstract：:New studies have shown that folding of beta-sheet proteins can occur with and without intermediates, with fast to slow refolding rates and late to very late transition states. These experiments demonstrate that, despite early speculation to the contrary, beta-sheet protein folding does not appear to be fundamentally d...

journal_title：Current opinion in structural biology

authors： Capaldi AP,Radford SE

更新日期：1998-02-01 00:00:00

abstract：:The recently determined structure of octameric mitochondrial creatine kinase has provided new insights into the functioning of this enzyme and its role in channelling energy from the mitochondria to the cytoplasm. Creatine kinase, a member of the family of guanidino kinases, is structurally similar to glutamine synthe...

journal_title：Current opinion in structural biology

authors： Kabsch W,Fritz-Wolf K

更新日期：1997-12-01 00:00:00

abstract：:Crystallographic studies of a number of aminoacyl-tRNA synthetases and their complexes with ATP, amino acid and cognate tRNA are leading to an increasingly detailed picture of how these sophisticated enzymes function. Within the two distinct structural classes of ten synthetases, many common features are apparent, alt...

journal_title：Current opinion in structural biology

authors： Cusack S

更新日期：1997-12-01 00:00:00

abstract：:Among the crystal structures of lectins determined recently, three--snowdrop lectin, jacalin and amaranthin--represent new lectin families. Their polypeptide folds share remarkably similar features and consist exclusively of beta structure. Autonomously folded beta-sheet subdomains, inter-related by a pseudothreefold ...

journal_title：Current opinion in structural biology

authors： Wright CS

更新日期：1997-10-01 00:00:00

abstract：:Recent advances in neutron and X-ray sources and instrumentation, new and improved scattering techniques, and molecular biology techniques, which have permitted facile preparation of samples, have each led to new opportunities in using small-angle scattering to study the conformations and interactions of biological ma...

journal_title：Current opinion in structural biology

authors： Trewhella J

更新日期：1997-10-01 00:00:00

abstract：:Metal-binding sites have been engineered into both de novo designed and naturally occurring proteins. Although the redesign of existing metal-binding sites in naturally occurring proteins still offers the most promise for a successful design, the more challenging goal of engineering metal-binding sites in de novo desi...

journal_title：Current opinion in structural biology

authors： Lu Y,Valentine JS

更新日期：1997-08-01 00:00:00

abstract：:Channel-forming bacterial toxins undergo a series of remarkable changes in solubility, oligomerization state, structure and dynamics during the processes of membrane binding, assembly, membrane insertion and channel formation. Recent high-resolution crystal structures of channel-forming toxins, in both water-soluble a...

journal_title：Current opinion in structural biology

authors： Gouaux E

更新日期：1997-08-01 00:00:00

abstract：:If protein structure prediction methods are to make any impact on the impending onerous task of analyzing the large numbers of unknown protein sequences generated by the ongoing genome-sequencing projects, it is vital that they make the difficult transition from computational 'gedankenexperiments' to practical softwar...

journal_title：Current opinion in structural biology

authors： Jones DT

更新日期：1997-06-01 00:00:00

abstract：:Prediction and modeling of RNA structures has become an indispensable tool of biological research disciplines. Currently, reliable predictions require massive input of experimental data. Structure-forming elements are conventional base pairs, as well as a rapidly increasing repertoire of novel structural motifs. New d...

journal_title：Current opinion in structural biology

authors： Schuster P,Stadler PF,Renner A

更新日期：1997-04-01 00:00:00

abstract：:Can a stereochemical recognition code explain sequence-specific protein-nucleic acid interactions? Whereas a code that is generally applicable to DNA-binding proteins of all known structural families is unattainable, the indications are that a code can describe at least some of the interactions of classical zinc finge...

journal_title：Current opinion in structural biology

authors： Choo Y,Klug A

更新日期：1997-02-01 00:00:00

abstract：:The three-dimensional structures of several biotin-binding proteins are now known, giving insights into the molecular architecture of the binding sites for biotin. In combination with biochemical and computational approaches, these structural insights provide the basis for our present understanding of biotin-protein i...

journal_title：Current opinion in structural biology

authors： Lindqvist Y,Schneider G

更新日期：1996-12-01 00:00:00

abstract：:Advances in the crystallization of biological macromolecules have come not only from the application of biochemical, molecular biological and immunological principles and techniques, but also from continued efforts to understand the crystallization process. Developments in crystallization methodologies, protocols, and...

journal_title：Current opinion in structural biology

authors： Gilliland GL,Ladner JE

更新日期：1996-10-01 00:00:00

abstract：:Recent studies have revealed a correlation between amino acid sequences around glycosylation sites in proteoglycans and the ability of cells to initiate and process glycosaminoglycan chains. Initiation depends on Ser-Gly/Ala dipeptides that have one or more acidic amino acids in close proximity. The formation of hepar...

journal_title：Current opinion in structural biology

authors： Esko JD,Zhang L

更新日期：1996-10-01 00:00:00

abstract：:H+ transporting, reversible F-type ATPases (ATP synthases) and V-type ATPases share major structural features and function by similar mechanisms. Recent structural and genetic experiments provide new insights into the organization of the transmembrane and coupling sectors of the enzymes and the molecular mechanics of ...

journal_title：Current opinion in structural biology

authors： Fillingame RH

更新日期：1996-08-01 00:00:00

abstract：:Recent advances in algorithmic techniques and statistics of sequence alignment have helped to make near-optimal alignments an important tool in the delineation of biologically valid alignments. Near-optimal alignments serve to judge the reliability of portions of a sequence alignment. The statistical assessment of sub...

journal_title：Current opinion in structural biology

authors： Vingron M

更新日期：1996-06-01 00:00:00

abstract：:In vitro selection experiments are now routinely used to identify functional nucleic acid residues and structures, and have become a tool for studying molecular recognition, molecular biology, and molecular evolution. Technical innovations that have been made during the past year include the use of modified monomers t...

journal_title：Current opinion in structural biology

authors： Uphoff KW,Bell SD,Ellington AD

更新日期：1996-06-01 00:00:00

abstract：:The past decade has witnessed the development of numerous systems for the presentation of antigens on the surface of self-assembling macromolecules. Although the sites for insertion were initially chosen empirically, the determination of the three-dimensional structures of a number of carrier macromolecules has enable...

journal_title：Current opinion in structural biology

authors： Lomonossoff GP,Johnson JE

更新日期：1996-04-01 00:00:00

abstract：:Protein design efforts are beginning to yield molecules with many of the properties of natural proteins. Such experiments are informed by and contribute to our understanding of the sequence determinants of protein folding and stability. The most important design elements seem to be the proper placement of hydrophobic ...

journal_title：Current opinion in structural biology

authors： Cordes MH,Davidson AR,Sauer RT

更新日期：1996-02-01 00:00:00

abstract：:In the past few years, structural biology has begun to reveal details of some of the receptors and associated interactions responsible for protein-mediated recognition events at the cell surface. Recent data span the fields of cytokine-receptor interactions, cell adhesion molecules and viral infection. ...

journal_title：Current opinion in structural biology

authors： Stuart DI,Jones EY

更新日期：1995-12-01 00:00:00

abstract：:The past year has witnessed a tremendous increase in our understanding of the structures and interactions of the GTPases. The highlights include crystal structures of G alpha subunits, as well as the first complex between a GTPase (Rap1A) and an effector molecule (c-Raf1 Ras-binding domain). In the field of elongation...

journal_title：Current opinion in structural biology

authors： Hilgenfeld R

更新日期：1995-12-01 00:00:00

abstract：:Progress in the synthesis, purification and crystallization of RNA has resulted in the determination of several X-ray crystal structures of RNA molecules over the past few years. Methods proven and under development will lead to future structure determinations and shed light on the structural basis for RNA's many func...

journal_title：Current opinion in structural biology

authors： Lietzke SE,Barnes CL,Kundrot CE

更新日期：1995-10-01 00:00:00